![]() ![]() Additionally, we demonstrate properties of individual routines and illustrate which reconstruction task they are suitable for, and evaluate the efficiency of a subset of the algorithms implemented. ![]() To develop the framework we reviewed and selected workflows which accomplish versatile reconstruction tasks according to today’s best practices. To perform such tasks in a streamlined way we introduce the Possum framework, software for reconstruction of three-dimensional images of the brain from series of two-dimensional images of stained sections. However, to compare or combine them with results from three dimensional imaging methods, reconstruction into a volumetric form is required. Techniques based on imaging serial sections of brain tissue provide insight into brain structure and function and the usage of complementary approaches is considered indispensable in characterizing the spatial organization of neuronal structures and their circuitry. Nencki Institute of Experimental Biology, Poland ![]()
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